6) CRISPR Cas9 – How to perform a CRISPR Knockin Experiment
6) CRISPR Cas9 – How to perform a CRISPR Knockin Experiment
Read our complete CRISPR Knockin Case Study here:
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Are you looking for a reliable and affordable way to knockin a gene? The CRISPR Cas9 system is the tool of the century for accomplishing targeted gene knockins.
In this video, the CRISPR Cas9 gene editing system is used to knockin the RFP gene into the AAVS1 Safe Harbor site in HEK Cells. The Safe Harbor site is a preferred site for gene knockins as it enables robust transgene expression with minimal toxic effects. First, we design an All-in-One CRISPR Cas9 sgRNA vector construct with the sgRNA targeted to the human AAVS1 locus. A donor plasmid containing the template for homology directed repair is also designed. After successful transfection, the success of the CRISPR Cas9 Knockin is verified using a PCR-based technique. This technique utilizes custom designed primers targeting a site within the RFP knockin. Knockin is confirmed when a PCR product is observed.
We welcome you to visit our website to learn more about our CRISPR Cas9 Knockin services as well as our full suite of CRISPR Cas9 services and products – designed to meet all your gene editing needs!
Read more about the basics of the CRISPR system on our knowledge base:
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Learn more about our CRISPR Knockout Cell Line Generation Service here:
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- CRISPR rivals put patents aside to help in fight against Covid-19 – STAT
- CRISPR 2.0: Base Editing in the Groove – Genetic Engineering & Biotechnology News
- The Promises of CRISPR Genome Editing in Biomedicine – Labiotech.eu
- The Code Breaker and Crispr People — the ethics of editing humanity – Financial Times
- ERS Genomics Licenses CRISPR Patents to CRO ZeClinics – GenomeWeb
- The Dark Side of CRISPR – Scientific American
- ERS Genomics and ZeClinics Sign CRISPR/Cas9 License Agreement – BioSpace
- Scientists Use CRISPR To Create Model of AML Progression – AJMC.com Managed Markets Network
- DECODR app identifies DNA mutations from CRISPR gene therapies – European Pharmaceutical Review
- CRISPR/Cas technology as a promising weapon to combat viral infections – Wiley
- Neanderthal-like ‘mini-brains’ created in lab with CRISPR – Nature.com
- Affordable CRISPR app reveals unintended mutations at site of CRISPR gene repair – Phys.org
- Affordable CRISPR app reveals unintended mutations at site of CRISPR gene repair – EurekAlert
- New CRISPR tech targets human genome’s complex code: Programmable CRISPR/Cas9-based kinase offers insights into, control over regulatory histone proteins – Science Daily
- After ditching Editas, AbbVie taps Caribou for new CRISPR, CAR-T pact – FierceBiotech
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