Conférence – E. CHARPENTIER – Le CRISPR Cas9 – La révolution de l’ingénierie génomique

Conférence – E. CHARPENTIER – Le CRISPR Cas9 – La révolution de l’ingénierie génomique

Conférence de Emmanuelle CHARPENTIER intitulée The CRISPR-Cas9 revolution in genome engineering: lessons learned from bacteria

00:00:00 Introduction
00:01:10 Understand diseases at the fundamental level
00:04:16 Jacques Monod (1910-1976)
00:05:30 Important milestones towards gene therapy
00:10:09 The CRISPR craze
00:10:56 Bacterial pathogens
00:11:26 Understand bacteria/host interactions
00:11:52 Infection of bacteria by invading genomes
00:14:14 The CRISPR-Cas adaptive immune system
00:17:37 – Spacer acquisition
00:19:04 – crRNA expression and maturation
00:20:06 – interference
00:23:01 – mecanisms
00:23:06 CRISPR-Cas has evolved into five major types
00:24:02 Sans titre
00:26:19 CRISPR-Cas9 technology: a two-component system
00:29:04 CRISPR-Cas9 pathway in bacteria
00:29:09 Type II CRISPR-Cas locus
00:29:30 Type II CRISPR-Cas: model of crRNA maturation
00:30:22 Type II CRISPR-Cas: model of DNA targeting
00:30:38 Type II CRISPR-Cas
00:30:40 CRISPR-Cas9: RNA-programmable DNA cleavage
00:31:08 Genome editing with sequence-specific nucleases
00:31:54 The CRISPR-Cas9 technology
00:34:01 Cas9 toolbox
00:34:17 tracrRNA is associated to type II CRISPR-Cas
00:34:46 Applications of CRISPR-Cas9 in biology
00:34:54 Applications of CRISPR-Cas9 in human medicine
00:37:10 CRISPR-Cas9: funding
00:37:24 CRISPR-Cas9: people
00:39:01 The Lab
00:39:08 Thank you
00:39:33 Questions

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