Gene Knockout using CRISPR, a Video Tutorial
CRISPR technology democratized for genome engineering. This game-changing breakthrough makes it feasible for every researcher to perform gene knockout without any major undertaking. To make things even easier, OriGene provides a pre-designed knockout kit for every human and mouse gene. Each kit includes all the plasmid constructs (2 targeting gRNAs, one scramble gRNA and a donor template vector) and a simple protocol. This video tutorial serves as a step-by-step guide for the gene knockout experiment.