5) CRISPR Cas9 – How to perform a CRISPR Knockout Experiment
5) CRISPR Cas9 – How to perform a CRISPR Knockout Experiment
Read our complete CRISPR Knockout Case Study here:
➜ https://goo.gl/EpEZDB
Due to CRISPR’s unparalleled ease-of-use and affordability, gene knockout experiments are now more feasible than ever before!
In this video, we will show you how we used the CRISPR Cas9 system to knockout the LIF gene in a colon carcinoma cell line. This is accomplished by the delivery of a Cas9 and sgRNA lentivector into the cell line followed by drug selection and monoclonal isolation. More importantly, we demonstrate how to characterize and verify your CRISPR Knockout using the PCR-based Surveyor Assay, Sanger Sequencing, and Next Generation Sequencing. This complete process of validation ensures that your final CRISPR Knockout cell line is 100% successful.
At the end of the video, we will introduce you to abm’s entire suite of CRISPR Knockout services – designed to help you jumpstart your Knockout experiments!
Read more about the basics of the CRISPR system on our knowledge base:
➜ https://goo.gl/df55ap
Learn more about our CRISPR Knockout Cell Line Generation Service here:
➜ https://goo.gl/EpEZDB
Watch more videos about CRISPR:
➜CRISPR Cas9 – Gene Regulation with dCas9: https://goo.gl/tRkHWd
➜ How a mini CRISPR Cas9 system is revolutionizing gene therapy! https://goo.gl/njRPv2
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- CRISPR rivals put patents aside to help in fight against Covid-19 – STAT
- CRISPR 2.0: Base Editing in the Groove – Genetic Engineering & Biotechnology News
- The Promises of CRISPR Genome Editing in Biomedicine – Labiotech.eu
- The Code Breaker and Crispr People — the ethics of editing humanity – Financial Times
- ERS Genomics Licenses CRISPR Patents to CRO ZeClinics – GenomeWeb
- The Dark Side of CRISPR – Scientific American
- ERS Genomics and ZeClinics Sign CRISPR/Cas9 License Agreement – BioSpace
- Scientists Use CRISPR To Create Model of AML Progression – AJMC.com Managed Markets Network
- DECODR app identifies DNA mutations from CRISPR gene therapies – European Pharmaceutical Review
- CRISPR/Cas technology as a promising weapon to combat viral infections – Wiley
- Neanderthal-like ‘mini-brains’ created in lab with CRISPR – Nature.com
- Affordable CRISPR app reveals unintended mutations at site of CRISPR gene repair – Phys.org
- Affordable CRISPR app reveals unintended mutations at site of CRISPR gene repair – EurekAlert
- New CRISPR tech targets human genome’s complex code: Programmable CRISPR/Cas9-based kinase offers insights into, control over regulatory histone proteins – Science Daily
- After ditching Editas, AbbVie taps Caribou for new CRISPR, CAR-T pact – FierceBiotech
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