CRISPR: Cas9 proteins search for DNA for gene editing

CRISPR: Cas9 proteins search for DNA for gene editing

In this UC Berkeley animation, several hundred Cas9 enzymes (red dots) search the nucleus of a live mammalian cell for a specific DNA sequence. They become white when they bind briefly before moving on. The lines shows the paths these enzymes have taken, color coded according to time. The Cas9 tracks show that the enzymes search by diffusing through the nucleus, and that off-target binding events are predominately short lived. Motion is slowed to half normal speed.

Video provided by Spencer Knight

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