CRISPR–Cas9 Structures and Mechanisms: Supplemental Video 2
A supplemental video from the 2017 review by Fuguo Jiang and Jennifer A. Doudna, “CRISPR–Cas9 Structures and Mechanisms,” from the Annual Review of Biophysics: http://www.annualreviews.org/doi/10.1146/annurev-biophys-062215-010822?utm_source=youtube&utm_medium=bb.doudna&utm_campaign=suppvideo
Shown: Structures of the Cas9–sgRNA bound to bona fide dsDNA substrates containing a canonical 5-TGG-3 PAM. This video highlights the path of the nontarget DNA strand in an active conformation with HNH nuclease domain positioned adjacent to the scissile phosphate within the target DNA strand. This is a conformation not observed in the ssDNA-bound and PAM duplex-bound structures and essential for concerted DNA cleavage.