Designing, Packaging & Delivery of High Titer CRISPR Retro & Lentiviruses via Stereotaxic Injection
An Open Access Protocol from JoVE – Neuroscience.
The CRISPR/Cas9 system offers the potential to make targeted genome editing accessible and affordable to the scientific community. This protocol is intended to demonstrate how to create viruses that will knockout a gene of interest using the CRISPR/Cas9 system, and then inject them stereotaxically into the adult mouse brain.
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