Episode 011 – CRISPR: Genome Editing Basics

Episode 011 – CRISPR: Genome Editing Basics

Life and Biology – Episode 11 – CRISPR: Genome Editing Basics
Dr. Joel Graff

In this video, I introduce genome editing and focus on how the CRISPR-Cas system can be used to precisely cut a genome in the
correct location.
Relevant links:
A single guide RNA can be formed with a linker connecting the crRNA and the tracrRNA:
http://www.sciencemag.org/content/337/6096/816.full.pdf (Charpentier/Doudna labs)

Proof of concept: Genome editing in human cells:
http://cdn.elifesciences.org/elife-articles/00471/pdf/elife00471.pdf (Doudna lab)
http://www.sciencemag.org/content/339/6121/819.full.pdf (Church lab)
http://www.sciencemag.org/content/339/6121/819.full.pdf (Zhang lab)