Posted by on January 28, 2018 3:59 am
Categories: Crispr

Dirty DNA is Okay – Sometimes! Obtaining Rapid PCR/RT-PCR Results from Diverse Sample Types

Whether you are confirming genomic alterations in transgenic mice or screening CRISPR-edited clones using T71E1 assays, sample preparation and PCR analysis can take a lot of time. It’s important to optimize each step of your assay to achieve an efficient workflow, reproducible results and reliable data. In this webinar we will review each step of end-point PCR/RT-PCR assays including (i) an investigation into potential contaminants in your sample, (ii) choosing the proper DNA or RNA extraction method, and (iii) identifying the best fitting amplification enzyme or master mix. We’ll also explore how to overcome assay development challenges in your research and discuss solutions that increase efficiencies in popular end-point PCR/RT-PCR applications.

Key Learning Objectives:

Discuss when to use extracted vs. purified nucleic acids in various applications
Identify the potential stumbling blocks in the PCR and RT-PCR workflows
Explore the utility of fool-proof PCR systems for all template types
Provide examples of quick and effective PCR screening applications

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