Tianjin station to generate the CRISPR/Cas9 genome editing technology research progress

Source: Tianjin station to generate the CRISPR/Cas9 genome editing technology research progress

The CRISPR/Cas9 as a revolutionary genome editing technique has been widely used in a variety of biological genome editing, the technology in specific applications, there are some problems, for example in the genome in the presence of“PAM-free”and“CRISPR-tolerant”and other areas cannot use the traditional CRISPR/Cas9 technology for editing; genome each locus editors are required to build a specific gRNA; the off-target phenomenon is also CRISPR/Cas9 technology urgent need to resolve the problem.

to solve the above problems, the Chinese Academy of Sciences, Tianjin industrial biotechnology Research Institute, researcher at BI Chang Hao led to the microbial synthesis biotechnology research team, the researcher Zhang Jimmy led the microbial metabolic engineering research team, successfully built without the new gRNA plasmid CRISPR/Cas9 efficient genome editing technology CAGO River. First, the one containing the special“N20PAM”sequence of edit segments by homologous recombination integrated into the genome, and then use the CRISPR/Cas9 with red homologous recombination technique, the insertion sites to achieve genome inside homologous recombination, thereby achieving genomic no trace of editing. The method may be in the genome of any sites to achieve a non-off-target editing, the editing process requires only the synthesis of one edit segment, and in General pCAGO plasmid with the help of it can complete the Edit, without having to build gRNA plasmid. The method of construction of synthetic biology research provides a simple and easy-tech means to reduce the CRISPR/Cas9 techniques use a threshold.

research work to obtain the national high-tech research and development program（863 program）and Tianjin, focus of special support, the relevant results are published in Scientific Reports.

papers link 

Figure 1. CAGO technical design and pCAGO structure diagram

Figure 2. CAGO genome editing schematic

Published at Fri, 06 Feb 2018 06:52:51 +0000