guideread:last refresh of Feng Zhang CRISPR magic shears in the recently launched new version of it. In holding fast and convenient, and other advantages on the basis of the lifting of the previous limitations, you can test multiple targets, and has the than ever up to 100 times the sensitivity. And through the test paper,“SHERLOCK”can achieve the visual observation of the test results.
nucleic acid features, fast and portable detection for human health is significant. Last year’s early, Zhang Feng team based on this proposed a named“SHERLOCK” (Specific High Sensitivity Enzymatic Reporter UnLOCKing) of the CRISPR diagnostic tools that make its fame.
The first generation of“SHERLOCK”can quickly detect RNA or DNA molecules, but there are still a lot of limitations. New Year, Feng Zhang led his team to we sent up anew“SHERLOCK”, it is possible to more quickly and accurately detect the sample of genetic characteristics, including pathogens and tumorDNA。and by a miniature paper test, and even able toachieve with the naked eye its results.
this results in a“Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science.” In 2 month 15, published in “Science”.
The research team developed a simple paper strip that can display a single gene feature detection results. The strip is immersed in the treated samples, there will be a thin line, indicating that the target molecule is to be detected. This feature can be in the outbreak during the outbreak brought a lot of convenience. At the same time,the new“SHERLOCK”has higher sensitivity, can be more accurately quantify a sample present in the target number and a plurality of test target.
“SHERLOCK”piece of paper to show us the synthesis of 兹卡病毒 and dengue Virus the presence of
leap off of the results
since no detection equipment, the“SHERLOCK”can be said to be closer to the field diagnosis. And because of its nucleic acid target detection on the versatility of the“SHERLOCK”the future wide range of uses.< b>it not only can be applied to health care, the diagnosis whether the patient is infected and the detection lead resistance or oncogenic mutation; also can be used for industry and agriculture, the monitoring of the supply chain, in order to reduce waste, improve safety, etc.
joint first author Jonathan Gutenberg(left)and Omar Abu da yeah(right)holding a note, to show us the“SHERLOCK”。
“SHERLOCK”:Cas13
Cas13 is a CRISPR-associated protein, can be used to bind specific RNA fragments, its target may be any gene sequence, including the viral genome, the drug-resistant genes even cancer mutation gene.“ SHERLOCK”the use of Cas13 positioning to a specific synthetic RNA and chopped after the release of signal molecules.
more of the detection target and the higher sensitivity
the previous“SHERLOCK”only���Measured a nucleic acid sequence, but the new version of“SHERLOCK”simultaneously to four different targets to provide a fluorescent signal. At the same time, through the use of another enzyme, the new version of“SHERLOCK”has higher sensitivity,improve100times, this breakthrough for the detection of the blood sample free of tumorDNAvital.
source: the translational medicine network.
original source: Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Feb 15. pii: eaaq0179. doi: 10.1126/science. aaq0179. [Epub ahead of print]
link: http://science.sciencemag.org/content/early/2018/02/14/science.aaq0179.full
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