The animal the better performance of the drug-induced CRISPR/Cas9 technology system

Source: the animal the better performance of the drug-induced CRISPR/Cas9 technology system

genes in space and time on the correct expression of its sophisticated orderly regulation of cell growth, proliferation, differentiation, senescence and apoptosis and other important physiological process of the orderly conduct of the premise and Foundation. Accordingly, by a controlled way, from the molecular level to achieve the gene function of precise control for the realization of biological mechanisms for a more accurate analysis and more controllable and more safe clinical application has very important significance.</ p>

Chinese Academy of Sciences, animal Research Institute Wang Yu Research Group is committed to the adoption of Chemical Biology tools development of novel and useful molecular and cell biology tools. The researchers proposed by the CRISPR/Cas9 system on the grafting of estrogen receptor elements ERT2 make it in cells within the approved bits by a small molecule drug 4-hydroxy-tamoxifen（4OHT-regulation, thereby establishing a drug-inducible CRISPR/Cas9 technology, HIT, Hybrid drug Inducible CRISPR/Cas9 Technologies, from the molecular level a more flexible and accurate control function of the event. Researchers from transcription activation system from the start, for a variety of technical programmes through a series of optimization, transformation, comparison, set up a low background, high efficiency of the drug-induced systems HIT-SunTag-in. On this basis, the use of different lengths of the sgRNAs can selectively guide the Cas9 protein to bind and cut the target DNA characteristics, to establish a set of can achieve drug-induced genome editing and transcription activation system HIT2, meaning “both”of. Further, they are back to back and the existing variety of drug-induced system, including Feng Zhang Laboratory of Split-Cas9 system, David Liu Laboratory of the intein system, etc. for comparison, the HIT-SunTag and HIT2 system exhibits lower background, higher efficiency, higher drug selection specificity, and reversibility and fast response, and many other advantages. These systems established, is more flexible, accurate, controllable editing and regulation of gene function, resulting in the analysis of key biological events to achieve higher spatial and temporal resolution provides a powerful technical tool, and then integrated play small-molecule drugs and gene therapy means of advantage, clinically oriented applications offers more potential.</ p>

associated research work to Multimode drug inducible CRISPR/Cas9 devices fortranscriptional activation and genome editing for the topic, published online in Nucleic Acids Research. Animal the master Lu Jia, Dr. Zhao Chen, Zhao Yingze, Zhang bid party for the papers Co-first author, researcher Wang Yu for papers communication to the author. Research work by the Ministry of Science and technology major national scientific research plan, National Natural Science Foundation of China, Chinese Academy of Sciences“hundred people plan”and stem cell and reproductive biology state key laboratory open project of the Fund for support.</ p>

drug-induced to achieve genome editing and transcriptional activation of the HIT2 system mechanism of action

Published at Fri, 08 Mar 2018 13:01:11 +0000