CRISPR SCREENING USING HIGH-RESOLUTION MELTING
New genome editing tools such as the CRISPR system have greatly expanded the ability to modify genes, including creating single nucleotide polymorphisms (SNPs) and small indels, in different models. But the time and resources required to screen large numbers of cells to identify gene edited samples is a considerable bottleneck.
High-resolution melting (HRM) measures temperature variations with high precision as the DNA strands melt, with mutations being detected by analyzing melt curves. HRM limits the amount of sequencing required at each step, reducing the amount of resources required for both screening and sample preparation.
Find out how HRM can ease the arduous process of CRISPR screening with this infographic from Canon Biomedical!
Published at Mon, 26 Mar 2018 13:46:59 +0000