Science:a major breakthrough! Scientists developed a super-fast CRISPR-Cas9 gene editing…

Science:a major breakthrough! Scientists developed a super-fast CRISPR-Cas9 gene editing…

2020 年 6 月 14 hearing /Bio ValleyBIOON/ –recently, a study published in the International JournalScienceon the study, from Johns Hopkins University and other Agency scientists through the study of the use of the photosensitive nucleotide developed a new method to accelerate the CRISPR-Cas9 gene editing process, the article, the researcher describes the entire experimental process, and this new method of precision; in the Science magazine of the same period wrote an Outlook article from the Memorial Sloan Kettering Cancer Center scientists have also outlined the CRISPR-Cas9 gene editing technology with the evolution of history.

Photo Credit: Ella Marushchenko

in the CRISPR-Cas9 gene editing process, enzymes Cas9 can be used as scissors in gene editing of specific sites to cut the DNA chain, while the guide RNA molecules it will help Cas9 enzymes binding to the desired chain DNA site, currently this process is part of the process takes a few hours to complete this study, researchers to the entire process is shortened to a few seconds time.

in the article, the researchers by adding a photosensitive nucleotide molecules to change the portion of the guide RNA sequence, which will block the guide RNA in light is introduced before the completion of its work, once the researchers introduced a light, the binding process will be in a few seconds occurred, the researchers this method will be referred to as“the cage of the French,”The caged the approach, because of the guide RNA molecules will be limited until it is instructed to complete its work, the researchers this method will be referred to as ultra-fast CRISPR gene editing means vfCRISPR it.

the researchers noted that delayed the editing process then quickly to activate it, which perhaps will be able to study in detail the process provides a certain possibility, while this new method can also improve gene editing precision, and allows one to edit a single allele; at the same time, this new method also could help researchers develop a heterozygous mutation, and a new method to study complex characteristics, and finally the researchers Medhi said in the article we describe how a CRISPR-Cas9 tools from a passivation of the tool into a precise tool, vfCRISPR technology seems to be a transformative advancement of science, because it can help researchers better understand gene editing process involved in double-strand break the cell kinetics of the reaction changes. （生物谷Bioon.com

Origin:

Yang Liu,Roger S. Zou, Shuaixin He, et al. Very fast CRISPR on demand, Science (2020). DOI:10.1126/science. aay8204

Published at Mon, 15 Jun 2020 07:54:00 +0000