Posted by on July 7, 2020 7:01 am
Categories: Uncategorized

Yang force/Chen Jia was invited to published gene editing technology systematic review papers

recently, the Chinese Academy of Sciences Shanghai nutrition and Health Research Institute, Yang force with the Shanghai University of Science and technology of life science and Technology College Chen Jia Professor, invited to speak at the internationally renowned academic journalTrends in Biochemical Sciencepublished entitled“A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing”of the review paper, from a brand new perspective on CRISPR/Cas genome editing technology to create and apply a comprehensive summary, and the field of the future possible direction of development for the Outlook.

human genomic DNA contains a human life activities necessary for genetic information, and human health are closely related, and genomic DNA abnormalities, including the nucleotide mutation is able to cause human diseases. The use of genome editing techniques you can correct the genomic abnormality in the DNA and thus achieve the treatment of human genetic diseases purposes. Typically, the genome editing system requires two main configuration components to achieve accurate genome DNA editing, an identification of the genomic DNA of a particular position of the positioning sub – (locator)and a specific genomic DNA was subjected to catalytic operation the operation of the sub – (effector in. For example, early genome-editing system for ZFN and TALEN, respectively, the ZF or TALE as a locator with Fok IDNA endonuclease as effector binding, may be of the genomic DNA to operate. In recent years, the use of the bacterial CRISPR/Cas immune system the development of a new generation of genome editing technologies in basic science and applied research aspects have made a series of revolutionary breakthrough. CRISPR/Cas itself has both with genomic DNA combination of locator domains, may have a cutting genomic DNA sequence of the effector domain of its simple operation, accuracy and efficiency of editing is high, and therefore in biomedical research is widely used. More importantly, in recent years, researchers in the CRISPR/Cas gene editing enzymes such as CRISPR/Cas9, the CRISPR/Cas12a, the CRISPR/Cas13 and the like with a nucleic acid deaminase editing enzyme such as cytosine deaminase APOBEC/AID, adenine deaminase mutant of TadA or ADAR, etc. and the reverse transcriptase, such as Moloney’s murine leukemia virus reverse transcriptase MMLV integration the development of a base editing system, Base Editor, BE and guide editing system PrimeEditor, PE, available in the single nucleotide level to achieve accurate and efficient targeting and gene editing. In this review article, the Yang Force researcher and Chen Jia Professor mainly based on CRISPR/Cas the new editing system the creation and application of a detailed comb; the combination of which in a correct human disease-related mutations applied to the new editing system strengths and weaknesses are summarized; for these editing systems the potential of genome and transcriptome mutation mechanisms are discussed, and presents possible future solutions.

Yang Force researchers engaged in Computational Biology and gene editing technologies research. Their study group recently with the Shanghai University of Science and technology Chen Jia Professor, team cooperation, and to clarify the APOBEC in CRISPR/Cas9-induced genomic DNA break repair generated during the mutation of the molecular mechanisms Leiet al., 2018, Nat Struct Mol Biol; and has successfully created four series of more than ten new base editing system, including reducing the by-product of the eBE system Wanget al., 2017, Cell Res, and can be in the high GC region and high methylation region for efficient base edit universal-type hA3A-BE system Wang et al., 2018, Nat Biotechnol and in A/T rich region for base editing dCpf1-BE(dCas12a-BE-a Li et al., 2018, Nat Biotechnol system and activate only background levels of DNA damage response pathways BEACON system Wang et al., 2020, Cell Rep), and it has been reported of a series of bases in the editing system to carry out the efficiency comparison and potential applications of the analysis( Wang et al., 2019, Genome Biol )。 During the study, Yang Force study group and the cooperative team also reported the use of base editing system for human genetic mutations to correct the prediction system BEable-GPS Base Editable prediction of GlobalPathogenic SNVs, and the prediction of whole-transcriptome RNA editing of the calculation analysis process RADAR(RNA-editing analysis pipeline to decode alltwelve types of RNA-editing events, also invited to post a series of gene editing-related comment and review paper Yang et al., 2019, CRISPR J; Chen, et al., 2019, Nat Biotechnol; Yang et al., 2019, Cell )。 Technology at

Figure 1. Cas9nickase as a locator and nucleic acid deaminase editing enzyme or reverse transcriptase used as effector combination constituting a series of efficient genome the new base editing system.

A, Cas9nickase with cytosine deaminase binding mediated C-to-T nucleotide Editing. B, Cas9 nickase with adenine deaminase mutant binding mediated A-to-G nucleotide Editing. C, Cas9 nickase with cytosine deaminase and adenine deaminase mutant binding at the same time mediated C-to-T and A-to-G nucleotide Editing. D, Cas9 nickase and in combination with reverse transcriptase mediated by arbitrary base change, a small fragment of the nucleotide insertion or deletion, etc.

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Published at Tue, 07 Jul 2020 11:00:13 +0000