Posted by on July 10, 2020 4:51 am
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CRISPR-assisted non-transgenic hybrids in food production

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hybrids in the past few decades greatly improved the world’s food crop production, but how efficient and stable production of hybrids has been in the field of Agriculture a major problem. Today, the Chinese Academy of agricultural Sciences experts in the use of gene editing technology to design a new breeding methods, using the latest gene editing the CRISPR technology, the R & D to establish a hybrid line of time reduction of 5 to 10 years. This technology also can produce non-genetically modified varieties.< span class="bjh-strong">the study in 7 on 8 November published in the Cell Press Cell Press’s JournalMolecular Plantthe molecules of the plant act on it.

in the grain growing areas, crossbreed than a purebred has more advantages. Will have different genetic characteristics of crop hybridization, the resulting offspring is usually higher than the first generation with superior traits such as high yield, high disease resistance and wide adaptability. This phenomenon is known as heterosis.< span class="bjh-br">

“but at this stage of hybrid seed production technology is very time-consuming.” Paper corresponding author, Chinese Academy of Agricultural Sciences, Xie Chuan Xiao and Professor Li Xin Hai said the Professor.

for example, hybrid seed production a crucial step is to find or produce that there is no effective pollen to the male sterile parent as the hybrid production of the mother in the present. This can largely avoid the mother present to accept their own pollen, and let them accept the other one has different traits of the crop of pollen, in order to achieve hybridization purposes. However, this infertility is characterized by the plant gene control, to develop a stable breeding sterile parental line usually takes a long time, but also need one to maintain fertility to maintain the system. Maintain Department production out of holding the system needs to be able to self-post production out of the holding line and sterile line, in order to a steady stream of breeding sterile line and maintain the system and the production of hybrids, the formation of efficient seed production technology for agricultural production to provide quality hybrid seeds of different kinds.

these steps for breeding of the parental gene has a high demand, production is also very complex. So much so that the cultivated hybrid species of the long cycle, low efficiency.

“these drawbacks are limitations of the existing hybrid instruments in agriculture more regular use.” Xie Chuan Xiao said,“Our new system, we can use CRISPR/Cas9 to simplify the process, as long as a conversion is enough.”

Xie Chuan Xiao, the team first constructed a equipped with the RNA-guided Cas9 enzyme expression vector。 This carrier is the purpose of the maize nuclear gene MS26 control of male fertility of important functional parts remove. They also created a second carrier, equipped with an artificial gene, MGM。 This gene has three functions: an energy recovery plant male flower development of the MS26 normal flowering traits, the one that makes pollen inactivation of the enzyme, as well as aLocated in the seed of the red fluorescent marker. The team will be the two vector is transformed into Agrobacterium, and mediated maize immature embryos for genetic transformation. Since corn is a spore plant, the transformed maize plants were screened carrying a pair of be edited MS26, as well as a separate MGM。 Edit after the corn plants from cross-breeding, will be in a 1 to 1 ratio to get two different generations: one is to only have infertility MS26 gene, the offspring, one that is both sterile MS26 and MGM offspring.

before a class no MGM offspring having a stable male-sterile gene, referred to as CMS, is very suitable for corn hybrid breeding and hybrid seed production of. And because CMS does not carry the foreign gene, use it with other non-transgenic varieties to produce the hybrids are not genetically modified crops.

the second class contains MGM gene of the offspring with its parent actually has the same genes, so this type of seed also known as holding lines. These plants can be self-cross-breeding, continuous production of sterile and maintain the system.< span class="bjh-br">

due to the MGM gene carried on the color of the mark protein, keep the lines of seeds in the fluorescent lamp will show a red can easily with the CMS distinguished, so that sorting of two types of seeds as possible.

“our system reduces the production of hybrid seed the cost of seed production without manual or mechanical emasculation is. So in this way the production of the seed price will fall, and seed quality can also be improved.” Xie Chuan Xiao said.

not only is the maize MS26 is also present in other food crops, including rice, millet, wheat and sorghum. So this technology can be directly applied to other crops, also can be used in other similar gene, such as rice on the MS7. span>

“as long as The Associated application licensing procedures do a good job, technically speaking, this system of large-scale application of the production does not have what too big obstacles. This type of technology in the United States and Japan and other countries and the ordinary crop of the same, belonging to the free of supervision types.” Xie Chuan Xiao said.< span class="bjh-br">

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Published at Mon, 10 Jul 2020 08:50:20 +0000