Posted by on July 13, 2020 9:27 pm
Categories: Baidu

Listeria monocytogenes protein provides the CRISPR kill switch

2020-07-14 09:10:52

from the soil commonly found in bacterial strains of a single protein will be for scientists to provide a more accurate RNA editing method.

Cornell University, Rockefeller University and Memorial Sloan·Kettering Cancer Center’s latest research, published in the 7 November 3 of the journal Science, the protein is referred to as AcrVIA1, you can terminate the CRISPR-Cas13 editing process.

co-author Martin Widmann(Martin Wiedmann)Dr. said:“We are extending the scientific Toolkit for the effective use of CRISPR and does not cause side effects.”’ 97 years, Gellert(Gellert)home food safety Professor, Cornell University(Cornell)Food Safety Laboratory and Milk Quality Improvement Program Director.“ Because of this bacteria, we have the opportunity to close and strengthen the RNA to change the capacity.”

CRISPR, or clustered regularly spaced Short Palindromic Repeat sequence, is a laboratory mechanism, can act like microscopic scissors like play a role, and precisely edit the DNA contained in the genes. In today’s use of six types of CRISPR, the CRISPR-Cas13 can edit RNA, to date, the RNA editing process in the lack of the suppression effect.

the scientists say, since the cause COVID-19 disease of the coronavirus SARS-CoV-2 is an RNA virus, and therefore this new edit attachments may be the coronavirus researchers useful.

the main author of the Alex Meeske is a Rockefeller University Professor and senior author Luciano Marraffini lab post-doctoral researchers, he suspected Listeria monocytogenes in protein(phage)may be RNA editing useful.

at the beginning of the study at the beginning of Meeske and food safety expert Wiedmann has made contact from his food pathogens was collected to obtain the genetic bacterial samples. Weidman laboratory PhD student Liao view autumn will study a range of from about 1,500 bacterial candidates is reduced to the 62 strains.

Wiedmann laboratory these samples were transferred to the Rockefeller, where interns Alice Cassel(Alice Cassel)of the 62 strains were sequenced, and the separation of the 20 candidate proteins.

A strain stand out: Listeria Seeligeri, A in the soil everywhere in the the harmless bacteria. Not like its powerful cousins(foodborne Listeria monocytogenes), which does not cause human disease.

Rockefeller University scientists found that from L. seeligeri protein AcrVIA1 immediately stopped the CRISPR editing process. Meeske said:“ AcrVIA1 in control Cas13 application is very useful. Any Cas13 edit something, this anti-CRISPR proteins can be off.”“ This is where you can be in the CRISPR editing process using the’kill switch’, it has become we can use the other tools.”

Widmann explained that scientists can now use a more rigorous means to study RNA problem. He said:“this tool makes us more accurate.”

Published at Tue, 14 Jul 2020 01:25:22 +0000