Posted by on July 19, 2020 11:01 pm
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Science blockbuster:a gene editing technique

the international journal Science published a study in the international journal Science published a study, scientists from Johns Hopkins University and other institutions, through research and the use of a photosensitive nucleotide, the development of new method the new method to accelerate the CRISPR-Cas9 gene editing gene editing CRISPR-Cas9 gene editing article the article of the Cas9 gene editing, researchers describes the entire experimental process and new method the new method of medium Sloan Cancer Center scientists also outlined the CRISPR-Cas9 gene editing technology development.

picture source:Ellamarushchenko

in the CRISPR-Cas9 gene editing process, the enzyme-Cas9-can be used as scissors in gene editing of a specific position of the cut DNA strand, while the guide RNA molecules will help the Cas9 enzyme with the expected chain in the DNA binding site, at present, part of the process may take several hours to complete, in this study, the researchers may be the entire process is shortened to a few seconds.

in the article, the researchers by adding a photosensitive nucleotide molecules to change the RNA sequence part of these molecules to block the orientation of the RNA and in the light of the introduction before the completion of his work, once the researchers introduced a light, the binding process will be completed within a few seconds, the researchers said this method is“Cage the Sino-French”is caged, because the orientation of the RNA molecule is instructed to complete its work before is limited, researchers this method will be referred to as ultra-fast CRISPR gene editing means vfCRISPR it.

the researchers noted that the delay in the editing process, and then quickly activate his might for a detailed study of the editing process offers some possibilities, at the same time, this new method can improve gene editing precision, and allows one to edit a single allele, at the same time, this new method can also help researchers to develop hybrid zygote mutations and a new way to study complex features, according to Medhi, in the article, we describe how a CRISPR-Cas9 tools from a passive tool into precise tools, vfCRISPR technology seems to be a revolutionary scientific advances, because he can help the researchers to better understand the gene editing process, double-strand breaks involved in the cell reaction kinetics.

Published at Mon, 20 Jul 2020 02:50:17 +0000