Posted by on July 21, 2020 10:36 pm
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knockout CRISPR stem cell research breakthroughs the latest progress-source well Bio

from the CRISPR system of gene editing potential to develop up, in 2013 began to practice since they have fundamentally changed the way we manipulate gene knockout capability, and has laboratories around the world has been widely used. When this gene editing capabilities with stem cell proliferation potential when combined together, scientists will improve their On Cell Biology, Human Genetics and Developmental Biology, and Regenerative Medicine of the future potential of the development of understanding.

since the CRISPR/Cas9 system, a next-generation genome sequencing and stem cell technologies Mature, they use a combination of the likelihood of also been inspired. The following is the rapid development of the field of the latest developments, to show in gene editing such as gene knockout, gene knock-in and point mutations, etc. stem cell research cross the field in a break of the latest progress.

modified gene-specific capabilities for the characterization of the genes of powerful functions, carried out the gene therapy, to correct a specific mutation, disease eradication, the engineered cells and organisms in order to achieve more innovative features and to obtain transgenic animals as disease models provides a powerful tool. Tufted at regular intervals the technical tedious sequence of CRISPR/Cas9 technology has revolutionized genome engineering. The CRISPR/Cas9 system in stem cell research enables researchers to develop new disease models to discover new therapeutic tool and solution to degenerative disease. In addition, with regard to the use of CRISPR/Cas9 as a new medical therapy invention of useful technologies challenges and future prospects.

1, theusingCRISPR/Cas9create a fight IL-1- /TNF-alpha-mediated inflammation, and stem

in order to create the ability to automatically adjust the stem cells or primary cells, in order to fight tenacious of IL-1 and TNF-α-mediated inflammation and stem cells, the researchers used a genome editing system CRISPR/Cas9 is. Encoding the Firefly luciferase transcriptional reporter gene or a cytokine antagonist of the transgene, either murine IL-1Ra or chimeric human sTNFR1-mouse immunoglobulin G Bloquel et al., 2004, have been targeting the murine iPSCs cells in the Ccl2 start codon diekmen’s log, etc, 2004 use the CRISPR/Cas9 platform.

the results show that genome engineering can be successfully applied to endogenous cells of the loop of re-connection, in order to achieve the inflammation of the medium and its antagonist between a predetermined input/output relationship. This is by quick response automatic control system for cell-based drug delivery or Cell-Based Vaccine Foundation. Stem cells inherent in cell signaling pathways of customization, but also to a variety of diseases to provide a more effective treatment method, innovative ideas.

2, thein a pluripotent or pluripotent stem cells genetically modified using theCRISPR/Cas9system

Binding of stem cells pluripotent, this technique represents a powerful tool that can generate for Disease Modeling, drug screening, toxicology and targeting therapy of a variety of cell types. Typically, the CRISPR/Cas9 system has been applied to the multipotent or pluripotent stem cells genetic modification, after which the cells differentiate into specific cell types, and for the functional analysis or clinical transplantation. CRISPR/Cas9 technology the recent advances have expanded stem cell research and its therapeutic application range. This article is a comprehensive overview of the CRISPR/Cas9 technology current applications and future prospects, especially in Stem Cell Research and therapy.

3 andbindingiPSCandCRISPR/Cas9technology research of genetic diseases molecular and cellular mechanisms

in the mRNA levels of the control gene RNAi on the contrary, the CRISPRi allows at the transcriptional level to suppress gene. This allows researchers to suppress the stem cells of some genes and deciphering their function. Kampmann explains:“for CRISPRi, we’ll transcriptional repressor domain, KRAB domain, targeted gene’s transcription start site to inhibit its expression. This knockout method is very effective, and the lack of RNAi-based gene knock-out of infamous off-target effects using the CRISPR/Cas9 system for gene knockout, Gene Knockout and point mutation-mediated iPSC stem cells contribute to the study of genetic disease mechanisms, including immune, metabolic, blood, neurodegenerative diseases and heart disease.

gene knock in addition to the cutting efficiencynot only can let a person have to generate protein glycosylation spectrum and the establishment of the regulation recording capacity, and has a variety of advantages, is a particularly attractive recombinant protein expression system. First of all, it is in the glutamic acid on the carboxyl group in tyrosine on the sulfation. Second, the operation is simple, by transient gene expression can rapidly produce recombinant proteins. Third, can be used for stable recombinant protein production. Some researchers use gene cells knock-out of the cutting efficiency of the system to produce gene edited cell lines, for GLUL genomic loci for targeted sequencing, to produce the EPO in stably transfected cell lines, and found that recombinant erythropoietin in the human body within the stable expression mechanisms.

the source of the well creatures according to customer needs, combined with the target genes for gene stably transfected knockout programme design.

scenario 1: a small fragment of the gene knockout program, the gRNA located in exon 2 at both ends of the intron, the knock except for the exon encoding the nucleotide number of non-3 ratio, the knock can be caused by shift code.

Scenario 2: frameshift gene knockout program, the gRNA located in the exon, the missing base number is for the non-3 multiples, knockout can occur frameshift mutation.

Scenario 3: a large fragment of the gene knockout program, the entire gene coding sequence knockout, to achieve a large fragment of the knockout effect.

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Published at Tue, 21 Jul 2020 23:09:42 +0000